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. 2017 Jun 9;8:15754. doi: 10.1038/ncomms15754

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative kymography of DsRed-mito and patterns of CoMIC (right diagram) in DN-Drp1+shMic60-expressing neurons. (b) Probability of CoMIC in elongated mitochondria from DN-Drp1+shMock- and DN-Drp1+shMic60-expressing neurons (94 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (*P<0.05 by Mann–Whitney rank sum test). Error bar indicate s.e. (c–f) Representative kymographies of mito-GFP and patterns of CoMIC (right diagram) in DN-Drp1+Mock (c), DN-Drp1+Mic60- (d), DN-Drp1+S-Opa1- (e) and DN-Drp1+S-Opa1+Mic60-expressing neurons (f). (g) Probability of CoMIC in elongated mitochondria from DN-Drp1-, DN-Drp1+Mic60- (118 mitochondria), DN-Drp1+S-Opa1- (71 mitochondria) and DN-Drp1+S-Opa1+Mic60-expressing neurons (63 mitochondria), and quantification of the total duration, frequency and average single duration of CoMIC (***P<0.01, **P<0.02 and *P<0.05 by Mann–Whitney rank sum test).