Table 1. Lifespan data Related to Figure 2.
| Experiment | Strain | Mean survival | SEM | Deaths | Total worm number | Relative to control | Relative to wt | ||
|---|---|---|---|---|---|---|---|---|---|
| % Lifespan change | p-Value | % Lifespan change | p-Value | ||||||
| # 1 | wt | 20.56 | 0.40 | 151 | 180 | N/A | N/A | N/A | N/A |
| daf-2 set, graphed in Fig 2A | mbk-1(-) | 18.40 | 0.27 | 180 | 200 | −10.51 | <0.0001 | −10.51 | <0.0001 |
| hpk-1(-) | 15.55 | 0.25 | 174 | 200 | −24.37 | <0.0001 | −24.37 | <0.0001 | |
| daf-2(-) | 49.40 | 1.20 | 173 | 200 | N/A | N/A | 140.27 | <0.0001 | |
| daf-2(-); mbk-1(-) | 42.47 | 1.06 | 207 | 210 | −14.03 | <0.0001 | 106.57 | <0.0001 | |
| daf-2(-); hpk-1(-) | 29.09 | 0.83 | 196 | 200 | −41.11 | <0.0001 | 41.49 | <0.0001 | |
| # 1 | wt | 21.50 | 0.41 | 123 | 200 | N/A | N/A | N/A | N/A |
| glp-1 set | mbk-1(-) | 19.21 | 0.28 | 200 | 200 | −10.65 | <0.0001 | −10.65 | <0.0001 |
| hpk-1(-) | 15.37 | 0.28 | 196 | 200 | −28.51 | <0.0001 | −28.51 | <0.0001 | |
| glp-1(-) | 26.39 | 0.54 | 154 | 200 | N/A | N/A | 22.74 | <0.0001 | |
| glp-1(-); mbk-1(-) | 20.37 | 0.36 | 166 | 200 | −22.81 | <0.0001 | −5.26 | 0.0008 | |
| glp-1(-); hpk-1(-) | 22.88 | 0.36 | 190 | 200 | −13.30 | <0.0001 | 6.42 | 0.3459 | |
| # 2 | wt | 16.94 | 0.22 | 136 | 200 | N/A | N/A | N/A | N/A |
| mbk-1(-) | 17.80 | 0.20 | 220 | 220 | 5.08 | 0.0888 | 5.08 | 0.0888 | |
| hpk-1(-) | 12.72 | 0.14 | 176 | 200 | −24.91 | <0.0001 | −24.91 | <0.0001 | |
| glp-1(-) | 24.14 | 0.62 | 187 | 240 | N/A | N/A | 42.50 | <0.0001 | |
| glp-1(-); mbk-1(-) | 20.81 | 0.18 | 360 | 400 | −13.79 | <0.0001 | 22.85 | <0.0001 | |
| glp-1(-); hpk-1(-) | 20.32 | 0.43 | 167 | 180 | −15.82 | <0.0001 | 19.95 | <0.0001 | |
| # 3 | wt | 20.76 | 0.35 | 199 | 220 | N/A | N/A | N/A | N/A |
| mbk-1(-) | 19.63 | 0.31 | 239 | 250 | −5.44 | 0.0028 | −5.44 | 0.0028 | |
| hpk-1(-) | 13.68 | 0.23 | 165 | 200 | −34.10 | <0.0001 | −34.10 | <0.0001 | |
| glp-1(-) | 22.23 | 0.46 | 150 | 200 | N/A | N/A | 7.08 | 0.0025 | |
| glp-1(-); mbk-1(-) | 18.39 | 0.15 | 234 | 300 | −17.27 | <0.0001 | −11.42 | 0.0028 | |
| glp-1(-); hpk-1(-) | 20.27 | 0.44 | 184 | 200 | −8.82 | 0.0013 | −2.36 | <0.0001 | |
| # 4 | wt | 19.12 | 0.42 | 170 | 200 | N/A | N/A | N/A | N/A |
| glp-1(-) | 24.72 | 0.70 | 181 | 200 | N/A | N/A | 29.29 | <0.0001 | |
| glp-1(-); mbk-1(-) | 17.83 | 0.46 | 129 | 150 | −27.87 | <0.0001 | −6.75 | 0.0192 | |
| glp-1(-); hpk-1(-) | 19.45 | 0.64 | 99 | 150 | −21.32 | <0.0001 | 1.73 | 0.6228 | |
| composite | wt | 19.83 | 0.22 | 458 | 620 | N/A | N/A | N/A | N/A |
| combined glp-1 sets from # 1/2/3, graphed in Fig 2B | mbk-1(-) | 18.89 | 0.16 | 659 | 670 | −4.74 | 0.0004 | −4.74 | <0.0001 |
| hpk-1(-) | 13.98 | 0.14 | 537 | 600 | −29.50 | <0.0001 | −29.50 | <0.0001 | |
| glp-1(-) | 24.26 | 0.33 | 491 | 640 | N/A | N/A | 22.34 | <0.0001 | |
| glp-1(-); mbk-1(-) | 19.97 | 0.13 | 760 | 900 | −17.68 | <0.0001 | 0.71 | 0.0181 | |
| glp-1(-); hpk-1(-) | 21.21 | 0.24 | 541 | 580 | −12.57 | <0.0001 | 6.96 | 0.0049 | |
The effect of the mbk-1(pk1389) and hpk-1(pk1393) loss of function mutations on lifespan relative to mbk-1(+) and hpk-1(+) animals was examined in different genetic backgrounds. % change in lifespan and p-Values from Mantel-Cox-tests were calculated relative to mbk-1(+) and hpk-1(+) control animals of the same genetic background (wt, daf-2(-) or glp-1(-)), and relative to the wild-type strain N2E. Experiment #1, daf-2 set was plotted in Figure 2A, combined data for the glp-1 sets. Experiments 1, 2 and 3 were plotted in Figure 2B. Lifespan increases observed for glp-1 relative to wild-type are consistent with experiments in the literature [51, 63, 64].