TABLE 1.

Oligonucleotides used as primers for PCR amplification

Gene	Forward primer sequence (5′ to 3′)	Reverse primer sequence (5′ to 3′)	Expected product size (bp)	Reference or Genbank accession no.
blaPSE1	TTTGGTTCCGCGCTATCTG	TACTCCGAGCACCAAATCCG	150	7
blaTEM	GCACGAGTGGGTTACATCGA	GGTCCTCCGATCGTTGTCAG	310	7
blaCMY-2	ATGATGAAAAAATCGTTATGC	TTGCAGCTTTTCAAGAATGCGC	1143	37
aphA1-Iab	AAACGTCTTGCTCGAGGC	CAAACCGTTATTCATTCGTGA	500	12
aadA	GTGGATGGCGGCCTGAAGCC	AATGCCCAGTCGGCAGCG	528	19
aadA2	CGGTGACCATCGAAATTTCG	CTATAGCGCGGAGCGTCTCGC	250	AF071555
strA	CCTGGTGATAACGGCAATTC	CCAATCGCAGATAGAAGGC	548	19
tetA(A)	GCTACATCCTGCTTGCCTTC	CATAGATCGCCGTGAAGAGG	210	X61367
tetA(B)	TTGGTTAGGGGCAAGTTTTG	GTAATGGGCCAATAACACCG	659	18
tetA(G)	GCTCGGTGGTATCTCTGC	AGCAACAGAATCGGGAAC	464	S52437
sulI	CTTCGATGAGAGCCGGCGGC	GCAAGGCGGAAACCCGCGCC	435	31
Class 1 integron	GGCATCCAAGCACAAGC	AAGCAGACTTGACTGAT	Variablea	18
qacE	ATCGCAATAGTTGGCGAAGT	CAAGCTTTTGCCCATGAAGC	210	31

^aThe amplicon size of class 1 integron may be variable, depending on the sizes and the numbers of the gene inserts. However, if no resistance gene cassette is inserted, the expected size is 153 bp.