Figure 3. LACC1 amplifies PRR-induced cytokines.

MDMs were transfected with scrambled or LACC1 siRNA, and then treated for 24 h with (a) 100 μg ml⁻¹ MDP (n=4; similar results were observed in an additional n=18), or (b) 100 μg ml⁻¹ TriDAP, 10 μg ml⁻¹ Pam₃Cys, 0.1 μg ml⁻¹ lipid A or 5 ng ml⁻¹ flagellin (n=4; similar results were observed in an additional n=8). Cytokine secretion+s.e.m. Scr, scrambled. ***P<0.001; ^†P<1 × 10⁻⁴; ^††P<1 × 10⁻⁵; determined by 2-tailed t-test.