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. 2017 Jun 9;8:15818. doi: 10.1038/ncomms15818

Figure 1. Ash1l facilitates TGF-β-mediated Treg cell induction via promoting Foxp3 expression.

Figure 1

(a,b) Representative flow cytometry (a) and quantification (b) of the percentage of Foxp3 in the CD4+ T cells stimulated under iTreg cell-skewing conditions (with TGF-β) for indicated times. (c) mRNA expression of Foxp3 in the WT and Ash1l-silenced CD4+ T cells stimulated under iTreg cell-skewing conditions (with TGF-β) for indicated times. (d,e) Representative flow cytometry (d) and quantification (e) of the percentage of Foxp3 in WT and Ash1l-silenced CD4+ T cells transduced with Ash1l-fragment-expressing lentivirus (Lenti-Ash1l-F1, Lenti-Ash1l-F2, Lenti-Ash1l-F3 or Lenti-Ash1l-ΔN) and cultured under iTreg cell-skewing conditions (with TGF-β) for 3 days. (f) mRNA expression of Foxp3 in WT and Ash1l-silenced CD4+ T cells transduced and cultured as in d. Error bars represent s.d. Student's t test. *P<0.05, **P<0.01. Data are representative of three independent experiments (a,d) or are from three independent experiments (b,c,e,f; mean±s.d.).