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. 2017 Jun 12;8:15680. doi: 10.1038/ncomms15680

Figure 1. DAZL regulates the exit from pluripotency and entry into the late PGC stage.

Figure 1

(a) Immunofluorescent staining of DAZL, OCT4 and DAPI (nuclei) in 12, 16 and 20 W human fetal ovaries. A magnified image is shown on the upper right corner. Bar, 10 μm. Arrow: OCT4-positive cells; arrow head: DAZL-strong positive cells. (b) Percentages of OCT4-positive cells, and DAZL-strong positive cells in the 12, 16 and 20 W human fetal ovaries. n=500–800 cells counted in three different fields, error bar is s.d. (c) Relative mRNA expression levels of OCT4, NANOG, PRDM14 measured by qPCR in the control (Ctrl), DAZL-overexpressing (oeDAZL) and DAZL-silenced (shDAZL), hESCs differentiated from day 2 to day 8. n=2 from each time point (replicates from ∼100,000 cells), three independent experiments conducted, error bar is s.d., asterisks indicate statistical significance (P<0.05, Student's t-test) between the two samples at the same time-points. (d) Immunofluorescent staining of DAZL and OCT4 in the Ctrl and oeDAZL hESCs at day 6. (e) Western analysis of OCT4, NANOG and DAZL in the Ctrl and oeDAZL hESCs at day 6. (f) Immunofluorescent staining of DAZL and VASA in in the Ctrl and oeDAZL hESCs at day 6. (g) 3′-UTR Luciferase reporter assays of VASA, and SYCP3 transfected with wild-type or the two DAZL mutants carrying point mutations F84A and R115G. n=4, (four independent biological replicates per cell populations, ∼25,000 cells in each sample), and the experiment had been conducted three times, error bar is s.d., asterisks indicate statistical significance (P<0.05, Student's t-test) between the two samples.