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. 2017 Jun 12;8:15680. doi: 10.1038/ncomms15680

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Representative images of meiotic spreads and immunofluorescent staining of PRDM9 and γH2AX in control and induced hESCs. (b) Representative images of meiotic spreads and immunofluorescent staining of SYCP3 and γH2AX in control and induced hESCs. (c) Magnified image of the meiotic spreads and the immunofluorescent staining of PRDM9 and γH2AX in induced hESCs. Bar, 20 μm. (d) Magnified image of the meiotic spreads and immunofluorescent staining of SYCP3 and γH2AX in induced hESCs. Bar, 20 μm. (e) Magnified image of the meiotic spreads and immunofluorescent staining of SYCP3 and MLH1 in induced hESCs. Bar, 10 μm. (f) Percentages of PRDM9- and γH2AX-positive nuclei during in vitro-induced meiosis from day 6 to day 9, n>200 meiotic nuclei at each time point, error bar indicates s.d. (g) Percentages of nuclei with no SYCP3 (N), punctate SYCP3 (P) or elongated SYCP3 (E) during in vitro-induced meiosis at day 7, n>200 meiotic nuclei. At least three independent experiments were conducted for each set of meiotic spreads and immunostainings described above.