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. 2017 Jun 12;8:15680. doi: 10.1038/ncomms15680

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Schematic timelines and simplified protocols for the differentiation of the undifferentiated hESCs to the harvesting of the transplant from mouse kidney capsules. Red arrow: harvested transplant. Representative images of H&E staining of a kidney area next to the transplant (b), a control transplant containing differentiated hESCs without inducers (c), a transplant containing suspended FLCs as described in (d), and a magnified picture of FLCs in the transplant (e). Arrows: FLCs with primary follicle-like structures; double-headed-arrows: GV-like staining in the FLCs. (f) Immunofluorescent staining of NOBOX in the transplant showing colocalisation of NOBOX and nuclei in the middle of follicles. (g) Immunofluorescent staining of AMH in the transplant showing the AMH-positive cells localized to the surrounding cells of follicles. Bar, 50 μm in b–e,g; 10 μm in f. Three independent transplantation experiments were conducted.