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. 2017 Jun 12;8:15758. doi: 10.1038/ncomms15758

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Quantification of the hypocotyl lengths (upper) and root lengths (lower) of 3-day-old etiolated seedlings of Col-0 grown on vertically oriented MS medium supplemented with 1 μM ACC or 2 p.p.m. ethylene (ET) and/or 50 μM (+) or 100 μM (++) PZA. Bars represent the average length (±s.d.) of fifteen seedlings (Student's t-test, between PZA-treated and non-PZA-treated seedlings; **P<0.01). (b) Quantification of the relative ethylene production of 30 3-day-old etiolated seedlings and 5-day-old green seedlings of eto1-2 with 50 μM PZA compared to MS treatment. Bars represent the means (±s.d.) of five independent treatments (Student's t-test, between PZA-treated and non-treated seedlings; **P<0.01). (c) Quantification the relative ethylene production (compared to Col-0 under MS treatment) of 3-day-old etiolated seedlings of Col-0, acs octuple mutant, ctr1-1 and ein2-5 with the application of 10 μM ACC and/or 50 μM PZA for 24 h. Bars represent the means (±s.d.) of three independent treatments (Student's t-test, between PZA-treated and non-treated seedlings; **P<0.01).