Figure 4. Translocation of INO80 persistently displaces DNA from the H2A–H2B interface.

(a) Nucleosomes modified at amino-acid residue 53 of H2B were used to examine movements of DNA on the octamer surface at the edges of nucleosomes. Shown here are DNA movements at the longer extranucleosomal DNA proximal edge (entry site) of nucleosomes. DNA in nucleosomes without remodelling are cleaved 54 bps from the dyad axis and are labelled as 0. Shorter DNA bands appear due to DNA moving into nucleosomes and across residue 53 of histone H2B. Numbers beside the bands correspond to the number of nucleotides moved from the starting position. (b,c) The amount of DNA cleaved at the starting position (initial crosslinking) and the total amount of those bands that accumulate during remodelling (sum of all remodelled species) were plotted versus time for INO80 (b) and ISW2 (c). Error bars represent s.e.m. calculated from three technical replicates.