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. 2017 Jun 12;8:15800. doi: 10.1038/ncomms15800

Figure 4. Quantification of synaptic proteins in Lrfn2 KO mice.

Figure 4

(a) Expression levels of synaptic proteins in the S1 fraction from adult hippocampus. The protein levels were densitometrically measured after immunoblotting (WT, n=8; KO, n=9 from three independent experiments; *P<0.05, t-test). (b) Immunostaining of hippocampus using an anti–PSD-95 antibody. Scale bar, 500 μm. (c) Quantification of fluorescence signal intensities in CA1, CA3 and dentate gyrus (DG) (n=3 mice, means of two images for each mice; *P<0.05, t-test). Or, oriens layer; SR, radial layer; ML, lacunosum molecular layer; SL, stratum lucidum layer. (d) Immunostaining for PSD-95 in the CA1 radial layer. Confocal images at a higher magnification than the images shown in b. Scale bar, 20 μm. (e) Quantification of intensity, size and number of PSD-95 puncta (n=6 sections from three mice per genotype; *P<0.05, t-test).