Figure 5. Antagonizing USP1 by APC^Cdh1 at DNA damage sites is critical for BRCA1 recruitment.

(a,b). Representative images of BRCA1 (a) and RIF1 (b) IRIF in U2OS-Fucci cells transfected with the indicated siRNAs and expression vectors. The cells were irradiated with 2 Gy and recovered for 1 h before the immunostaining. (c,d) Quantification of BRCA1 (c, n>226) and RIF1 (d, n>195) focus formation in a,b. Blue box designates cells with more than 20 (BRCA1 positive) and 10 (RIF1 positive) foci, respectively. The percentages of BRCA1-positive and RIF1-positive cells are indicated above each box. Scale bars represent 10 μm.