FIG 3.

Detection of CheA1 in whole-cell lysates of A. brasilense wild-type strain Sp7 and its ΔcheA1 mutant derivatives expressing CheA1 and its variants from a plasmid by Western blotting. The mutant derivatives were expressing CheA1, variants with single-amino-acid residue replacements, or truncated variants of the protein from a medium-copy-number broad-host-range plasmid. A polyclonal antibody raised against CheA1ΔTMX was affinity purified as described in Materials and Methods prior to use. Each well is identified with the strain and with the parental or variant protein expressed from the broad-host-range vector in parentheses. The black lines indicate junctions separating lanes that were spliced from the original image in order to show samples run on the same gel in a single row.