TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristic(s) | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| Top10 | Cloning strain | Invitrogen |
| OMNImax | Cloning strain | Invitrogen |
| S17-1 | thi endA recA hsdR RP4-2Tc::Mu-Km::Tn7 | 25 |
| Azospirillum brasilense strains | ||
| Sp7 | Parental strain, wild type | ATCC (ATCC 29145) |
| Sp245 | Parental strain, wild type | 20 |
| ΔcheA1 mutant | Δ(cheA1)::gusA-Km (Km) | 17 |
| Δche1 mutant | Δ(cheA1-cheR1)::Cm (Cm) | 17 |
| ΔcheA1Sp245 mutant | ΔcheA1:Gm insertion in Sp245 | This work |
| Plasmids | ||
| pDONR 221 | Cloning vector | Invitrogen |
| Topo 2.1 | Cloning vector | Thermo Fisher |
| pBBR1MCS3 | Cloning vector | 23 |
| pBSKII(+) | Cloning vector | Stratagene |
| pRH005 | Gateway destination vector for C-terminal YFP fusion with protein of interest | 27 |
| pDONRTMX | pDONR221 carrying TMX | This work |
| pDONRCheA1 | pDONR221 carrying CheA1 | This work |
| pDONRCheA1ΔTMX | pDONR221 carrying CheA1ΔTMX | This work |
| pDONRTlp1 | pDONR221 carrying Tlp1 | This work |
| pBBRCheA1 | pBBR1MCS3 expressing CheA1 | 17 |
| pBBRCheA1ΔTMX | pBBR1MCS3 expressing CheA1 with the TMX domain deleted | This work |
| pBBRCheA1ΔREC | pBBR1MCS3 expressing CheA1 with the REC domain deleted | This work |
| pBBRTMX | pBBR1MCS3 expressing the TMX domain of CheA1 | This work |
| pRHCheA1 | pRH005 expressing CheA1 | This work |
| pRHTMX | pRH005 expressing TMX domain | This work |
| pRHCheA1ΔTMX | pRH005 expressing CheA1 with the TMX domain deleted | This work |
| pRHTlp1 | pRH005 expressing Tlp1 | This work |
| pKGmob-Gm | Mobilizable suicide plasmid | Lab strain |
| pKGmob-cheA1 | pKGmob-Gm expressing Sp245 CheA1 | This work |
| pBBRCheA1H252Q | pBBR1MCS3 expressing CheA1H252Q | Lab strain |
| pBBRCheA1D1055N | pBBR1MCS3 expressing CheA1D1055N | This work |
| pBSKCheA1 | pBSKII(+) carrying CheA1 | This work |
| pBSKCheA1ΔTMX | pBSKII(+) carrying CheA1 with the TMX domain deleted | This work |