TABLE 2.

Primers used in this study

Primer name	Sequencea
CheOp-HindIII-F	5′-CCCAAGCTTCAGCGCGATGAACTGGTTGGACC
CheOp-BamHI-Rev	5′-CGCGGATCCCGTAATCAGACGGTCCTGGTTAG
CheA1ΔTMX-F2	5′-GAAGATCTGCGGTCGCGCGGAATTC
CheA1ΔTMX-Rev	5′-GAAGATCTGACCGCGTCCGCCGCACG
CheA1promXhoI-F	5′-CCGCTCGAGCAGCGCGATGAACTGGTT
CheA1DNup HindIII-R	5′-AAGCTTCACGGCGGTGACGTC
Sp245cheA1-F	5′-GAATCACGTGACGTCGGA
Sp245cheA1-R	5′-CGACGCCGCGGCCCGACA
CheA1prom KpnI-F	5′-CGGGGTACCCAGCGCGATGAACTGGTTG
CheA1ΔREC XhoI-R	5′-CTCGAGTCATGGAGCGCCCTCTCC
GWCheA1-F	5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTCAGCGCGATGAACTGGTT
GWCheA1-R	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTTGCGGCACCTTTCTGCTCG
GWCheA1TMX-R	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTTTCCACGTCGAGCAACGAC
Tlp1GWFwd	5′-GGGGACAAGTTTGTACAAAAAGCAGGCTCCGGCATGCGGATCAGGGC
Tlp1GWRev	5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCCGGCCAGCCCACCGGGCGCCT

^aItalics indicate restriction enzyme sequences.