FIG 6.

InvS regulates the level of FimZ. (A) InvS downregulates fimZ-gfp expression in Salmonella. The 5′ UTR along with the full ORFs of FimZ and FlhD were translationally fused to GFP. Plasmids derived from pXG30, expressing GFP fusion proteins, were cotransformed with pBAD-derived plasmids expressing InvS or the vector control. Bacterial ICDH was detected using polyclonal anti-ICDH antibodies as the loading control. The levels of GFP fusion proteins were determined by Western blotting with polyclonal anti-GFP antibodies. (B) Quantification of GFP fusion proteins from three independent experiments. Values represent GFP fusion protein levels after normalization with that of ICDH. Data are representative of three experiments. The P values were calculated using the Student t test. (C) The expression of FimZ-GFP was decreased in the presence of InvS. pXG30-derived FimZ-GFP was transformed into the WT or the ΔinvS strain. Western blot of the 2-fold dilution series showing decreases of FimZ-GFP levels in the presence of InvS.