FIG 8.

InvS regulates flagellar expression. (A) InvS upregulates the expression of fliC. The expression of FliC protein was detected by Western blotting with monoclonal anti-FliC antibodies. Bacterial ICDH was detected using anti-ICDH polyclonal antibodies as the loading control. (B) Quantification of FliC expression. FliC expression in the WT strain was defined as 1. Values represent relative protein expression levels after normalization with that in the WT. Data are representative of three experiments. The P values were calculated using the Student t test. (C) Two-microliter samples of Salmonella cultures (optical density at 600 nm, 1.0) were inoculated onto LB plates made up of 0.3% Bacto agar (Difco), and cultures were grown at 37°C. Photos were taken 6 h postinoculation. (D) Halos around the colonies were measured after 6 h of incubation at 37°C. Data are representative of three experiments. The P values were calculated using the Student t test.