FIG 2.
Phenotypic characteristics of wild-type and gene-complemented E. coli strains. (A) Sensitivity of bacteria to oxidative stress, as measured by a hydrogen peroxide survival assay. Data are means ± standard errors of the means (SEM) (n = 3). (B) Beta-galactosidase (LacZ) activity of bacteria grown for 6 h on X-Gal LB agar in the absence and presence of IPTG. (C) Surface expression of type-1 fimbriae, as detected by enzyme-linked immunosorbent assay (ELISA) using an anti-FimH antibody. (D) Motility of bacteria grown on LB 0.2% agar for 6 h. For the gene complementation study, JJ1886 bacteria were transformed with a control (empty) plasmid (pE; pACYC184) or pACYC184 carrying rpoS, lacI, or lrhA from JJ1886 or JJ1887 (pRpoSJJ1886, pRpoSJJ1887, pLacIJJ1886, pLacIJJ1887, and pLrhAJJ1887, respectively).