FIG 5.
Effects of gene complementation on JJ1886 pathogenicity in mice. Mice were inoculated with JJ1886 bacteria transformed with an empty plasmid (pE; pACYC184) or pACYC184 carrying the rpoS, lacI, or lrhA gene from strain JJ1887 (pRpoSJJ1887, pLacIJJ1887, or pLrhAJJ1887). The percent change in the clinical status of each individual mouse (averaged over 3 days postchallenge) in each treatment group relative to the mean value for the control group (pE) is shown (normalized mean score). n, number of mice tested. Horizontal bars indicate the group means. Data shown are combined from three independent experiments. Significance of the difference in mean score between treatment groups and control was evaluated using simple linear regression (for pRpoSJJ1887 and pLacIJJ1887, using data obtained in one independent experiment each) or by multilevel mixed-effects linear regression, to adjust for random effects (for pLrhAJJ1887, using data from two independent experiments); n.s., not significant.