TABLE 1.

Genetic differences between household 1 strains JJ1886 and JJ1887^a

Locus tag	Chromosomal position	Genetic locusb	Function(s)	Genetic change (amino acid change)	Presence of genetic change in strain:
					JJ1886	JJ1887
P423_01815	400099–401058	lacI	LacI transcription factor	G890A (G297E)	+
P423_03060	666606–666815	cspE	Cold shock protein CspE	G140A (G47D)		+
P423_03335	717032–717919	dapA	Dihydrodipicolinate synthetase	G85A (V29M)		+
P423_07890	1590530–1591663	ompN	Outer membrane porin	C372T (SYN)	+
P423_09420	1916277–1917035	ydjF	DeoR-family transcription factor	G292A (A98T)	+
P423_12780	2598783–2599721	lrhA	LrhA transcription factor	C565T (L189F)	+
P423_15025	3056283–3057116	rpoS	RNA polymerase sigma factor RpoS	C140A (S47stop)	+
P423_19575	4002705–4004105	gadA	Glutamate decarboxylase	C717T (SYN)		+
Plasmid	NA	pJJ1886_4	Conjugative plasmid	Absence		+
Plasmid	NA	pJJ1887_5	R-factor plasmid	Absence	+
PAI	4907731–4949587	PAI	Iron acquisition system (Fec), antigen 43 adhesin (Ag43), hemolysin expression-modulating protein (Hha)	Duplication	+
P423_04930	1027744–1027875	NA	Phage hypothetical protein	IS66 insertion	+
NA	1447788–c	NA	Intergenic phage region	IS66 insertion	+
NA	NA	NA	NA	IS66 insertion upstream of PAI duplicate	+
NA	NA	NA	NA	IS66 insertion downstream of PAI duplicate	+

^aPAI, pathogenicity-associated island; NA, not applicable; SYN, synonymous; IS66, insertion sequence 66; +, genetic change present. Locus tags and chromosomal positions are given as in the reference JJ1886 genome.

^bFor those genes without a designated name in the JJ1886 annotation, the homolog designation available from annotations of other E. coli strains was used. Genes encoding transcriptional regulators are shown in bold.

^cThis chromosomal insertion of IS66 is not present in the originally published sequence of the JJ1886 genome.