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. 2017 Jun 15;37(13):e00029-17. doi: 10.1128/MCB.00029-17

FIG 6.

FIG 6

Cet1's NTD does not regulate recruitment of Spt5 or Spt6 to the promoter-proximal sites (or 5′ ORFs) of ADH1, PGK1, PYK1, and PMA1. (A and B) ChIP analysis of Spt6 at the 5′ ORFs of ADH1, PGK1, PYK1, and PMA1. (C and D) ChIP analysis of Spt5 at the 5′ ORFs of ADH1, PGK1, PYK1, and PMA1. (E and F) Analysis of Myc-tagged Spt16 and HA-tagged Paf1 association with the ADH1, PGK1, PYK1, and PMA1 coding sequences in the strain expressing HA-tagged Paf1 under the control of the GAL1 promoter (PGAL1-PAF1-HA) and Myc-tagged Spt16 after switching the carbon source in the growth medium from galactose to dextrose for 4 h. The error bars indicate SD.