TABLE 1.

Primer pairs used for PCR analysis in the ChIP assay

Targeta	Primer pair sequences
ADH1 (A)	5′-CTACACCAATTACACTGCCTCATT-3′, 5′-ACTTCAATAGATGGCAAATGGA-3′
ADH1 (B)	5′-GGTATACGGCCTTCCTTCCAGTTAC-3′, 5′-GAACGAGAACAATGACGAGGAAACAAAAG-3′
ADH1 (C)	5′-CTGCACAATATTTCAAGCTATACCAAGC-3′, 5′-GCCTTTGGCTTTGGAACTGGAATATC-3′
ADH1 (D)	5′-GATATTCCAGTTCCAAAGCCAAAGGC-3′, 5′-CTTAACTGGCAATGGCCAGTCAC-3′
ADH1 (E)	5′-CGGTAACAGAGCTGACACCAGAGA-3′, 5′-ACGTATCTACCAACGATTTGACCC-3′
ADH1 (F)	5′-GATATTCCAGTTCCAAAGCCAAAGGC-3′, 5′-CTTAACTGGCAATGGCCAGTCAC-3′
ADH1 (G)	5′-CTGGTTACACCCACGACGGTTCTT-3′, 5′-GCAGACTTCAAAGCCTTGTAGACG-3′
ADHI (H)	5′-CGGTAACAGAGCTGACACCAGAGA-3′, 5′-ACGTATCTACCAACGATTTGACCC-3′
PYK1 (5′ ORF)	5′-CTTGTTTCTATTTACAAGACACCAATC-3′, 5′-CTTTGGACCGATGGTACCAATG-3′
PYK1 (ORF or 3′ ORF)	5′-AAGTTTCCGATGTCGGTAACGCTAT-3′, 5′-TTGGCAAGTAAGCGATAGCTTGTTC-3′
PGK1 (5′ ORF)	5′-CAGATCATCAAGGAAGTAATTATC-3′, 5′-GTCAACTCTGATGAAGACACGC-3′
PGK1 (ORF or 3′ ORF)	5′-AGACGAAGTTGTCAAGAGCTCTGC-3′, 5′-GAAAGCAACACCTGGCAATTCCT-3′
PMA1 (A)	5′-TCGATGGTGGGTACCGCTTAT-3′, 5′-GATGTTAGACGATAATGATAGGAC-3′
PMA1 (B or 5′ ORF)	5′-ATCTTCTGACGATGACGATATCG-3′, 5′-TTCTGGAACTGGTCTAGCTTCA-3′
PMA1 (C or 3′ ORF)	5′-TCGGTGCTATGAACGGTATTATG-3′, 5′-ACATGGTAGCGATGATGTCGACAG-3′
GAL1 (UAS)	5′-CGCTTAACTGCTCATTGCTATATTG-3′, 5′-TTGTTCGGAGCAGTGCGGCGC-3′
GAL1 (Core)	5′-ATAGGATGATAATGCGATTAGTTTTTTAGCCTT-3′, 5′-GAAAATGTTGAAAGTATTAGTTAAAGTGGTTATGCA-3′
GAL1 (promoter-proximal site)	5′-ACCTCTATACTTTAACGTCAAGGAG-3′, 5′-TTTCGGCCAATGGTCTTGGTAA-3′
GAL1 (ORF1)	5′-GTGAGGAAGATCATGCTCTATACG-3′, 5′-GGCGGTTTCAAACTTGTTAGATAC-3′
GAL1 (ORF2)	5′-CAGAGGGCTAAGCATGTGTATTCT-3′, 5′-GTCAATCTCTGGACAAGAACATTC-3′
Chr V	5′-GGCTGTCAGAATATGGGGCCGTAGTA-3′, 5′-CACCCCGAAGCTGCTTTCACAATAC-3′

^aUAS, upstream activating sequence; Core, core promoter; ORF1 and ORF2, two different locations in the ORF; 5′ ORF, toward the 5′ end of the ORF; 3′ ORF, toward the 3′ end of the ORF. Different locations at a genomic locus are designated by different letters.