Strains |
|
|
E. coli
|
|
|
TOP10 |
Cloning strain |
Invitrogen |
INV110 |
dam and dcm methylase deficient |
Invitrogen |
M. morganii
|
|
|
G858 |
Clinical isolate, tigecycline MIC = 1 μg/ml |
This study |
G1492 |
Clinical isolate, tigecycline MIC = 4 μg/ml |
This study |
GC7676 |
G1492 insertion mutant, EZ::TN inserted in 453 bp of acrA
|
This study |
GC7677 |
G1492 insertion mutant, EZ::TN inserted in 946 bp of acrA
|
This study |
GC7743 |
GC7676 transformed with pCLL3444 |
This study |
GC7744 |
GC7677 transformed with pCLL3444 |
This study |
Plasmids |
|
|
pCR2.1-TOPO |
PCR cloning vector |
Invitrogen |
pUCGm |
pUC19 derivative containing gentamicin resistance cassette |
13 |
pCLL3443 |
pCR2.1-TOPO with cloned acrAB genes (4,859-bp PCR fragment) |
This study |
pCLL3444 |
pCLL3443 with cloned gentamicin cassette (878-bp XbaI fragment) |
This study |