TABLE 3.
Gene(s) | Product size (bp) | Purpose | Sequence (5′→3′) of:
|
||
---|---|---|---|---|---|
Forward primer | Reverse primer | Fluorescent probea | |||
acrAB | 4,859 | TOPO cloning | ATCCACTAATGACGTCGC | GCAGAGTAAATGCCTGAACG | NAb |
acrA | 72 | RT-PCR | ACCTGCGTCTGAAACAAGAAATC | CCAGGCTGACAGCAACTTTACC | CCAGCGGTGCCGTGGATAAAGAAC |
rrsE | 71 | RT-PCR | TTGACGTTACCCGCAGAAGAA | GCTTGCACCCTCCGTATTACC | TAACTCCGTGCCAGCAGCCG |
Labeled with 6-carboxyfluorescein (6′-FAM) at the 5′ end and with 6-carboxytetramethylrodamine (TAMRA) at the 3′ end.
NA, not applicable.