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. 2017 Jun 15;16:110. doi: 10.1186/s12934-017-0719-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic representation of the gene constructs used for αAI production. Diagrams of a the native αAI gene from cv. Pinto comprising signal peptide (SP), α- and β- subunits and the propeptide sequences; b the synthetic αAI-OPT gene, optimised for expression in two yeasts, including a Kozak consensus sequence and flanked by HindIII and NotI restriction sites. Diagrams of constructs c pKLAC2-αAI-OPT and d pYES2-αAI-OPT. P_Lac4-PBI and P_Gal1 correspond to promoters Lac4-PBI and Gal1 in pKLAC2 and pYES2, respectively. TT_Lac4-PBI and TT_CYC correspond to 3′ terminators Lac4-PBI and CYC in pKLAC2 and pYES2, respectively