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Antibody dot blot screening of culture supernatants of K. lactis YCT390 with αAI-OPT. Cleared supernatants (200 µL) of transformed colonies of K. lactis YCT390/αAI-OPT. Pure Pinto-αAI (200 ng) used as positive control (+). As negative controls, cleared culture supernatant (200 µL) of untransformed strain YCT390 (−) and culture media YPCGal (200 µL) without cells (− −). Circled dots correspond to dots with a signal intensity equivalent to that of positive control
