Fig. 4.

SDS-PAGE and western blots of proteins from selected lines of K. lactis YCT390 with αAI-OPT. a SDS-PAGE and b Western blot of culture supernatants (TCA-precipitated proteins from 5 mL) of strains 13B, 9B, 8B, 50A, 68A, 39B, 17A, 38B, 35A, 47B, 29B, 76A, 72A and 37B. c SDS-PAGE and d western blot of lysed-cell contents (25 µg of protein) of strains 13B, 9B, 8B, 50A, 68A, 39B, 17A, 38B, 35A, 47B, 29B, 76A, 72A and 37B. Untransformed strain YCT390 was used as a negative control (−C) and pure Pinto-αAI (200 ng) was used as a positive control (+C). Molecular markers are shown in kilodaltons at left