Fig. 5.

Inhibition of α-amylase by extracts from recombinant strains of K. lactis. Inhibition of porcine pancreatic α-amylase by a partially purified culture supernatants (300 µg of protein) and b lysed-cell extracts (500 µg of protein) from five colonies of K. lactis YCT390/αAI-OPT strains 13B, 68A, 17A, 47B and 29B. Untransformed strain YCT390 was used as negative control (−C) and pure Pinto-αAI (200 ng) was used as positive control (+C). Culture supernatants were partially purified with DEAE-Sepharose prior to inhibitor activity assays. Results are expressed as inhibition units (IU). Data from triplicate experiments were expressed as the average ± standard error