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. 2017 Jun 15;16:110. doi: 10.1186/s12934-017-0719-4

Table 2.

Purification of recombinant αAI from culture supernatants of K. lactis YCT390/αAI-OPT strain 68A

Purification step Total protein (μg) Specific inhibitory activity (IU/mg)
Dialysed and freeze-dried culture supernatant 899 ND
Anion-exchange batch-adsorption (DEAE-Sepharose CL-6B) 227 8
Affinity batch-adsorption (α-amylase-Sepharose) 8.45 234