Fig. 5.

Effects of IDO inhibitor on epithelial integrity, epithelial apoptosis, and tracheal occlusion in allograft mice with ASCs treatment. Tracheal grafts described above were harvested on days 14 and 28 post-transplant and fixed with paraformaldehyde. a Tracheal sections were stained with H&E. Percentages of epithelial loss was determined on day 14 post-transplant. b The transverse sections of tracheal grafts on day 14 were examined with TUNEL assay. Cells with dark brown staining nucleus were apoptotic cells. The graph represented average number of TUNEL positive cells in a section. c Percentages of tracheal occlusion were determined on day 28 post-transplant. All data are expressed as mean ± SEM; n = 5 per group. *, p < 0.05; **, p < 0.01 and ***, p < 0.001