Table 2.
Characteristics of the nSSRs markers used in the Iberian populations of Brachypodium distachyon
| Locus | Repeat motif | Allele size range (bp) | Na | pnull | AR | Ho | Hs | HT | Gst | FIS | FIS § |
|---|---|---|---|---|---|---|---|---|---|---|---|
| ALB006 | (GT)15 | 360–374 | 16 | 0.00399 | 3.318 | 0.000 | 0.051 | 0.614 | 0.917 | 1.000a | 1.000 |
| ALB022 | (CT)11 | 354–358 | 14 | 0.00464 | 2.909 | 0.000 | 0.000 | 0.520 | 1.000 | 1.000 | 1.000 |
| ALB040 | (CTT)8 | 176–182 | 15 | 0.00416 | 1.975 | 0.024 | 0.031 | 0.169 | 0.819 | 0.221a | 0.871 |
| ALB050 | (GT)15 | 217–231 | 19 | 0.00311 | 4.838 | 0.000 | 0.072 | 0.794 | 0.910 | 1.000a | 1.000 |
| ALB086 | (AAG)7 | 190–198 | 14 | 0.00434 | 2.909 | 0.000 | 0.000 | 0.520 | 1.000 | 1.000 | 1.000 |
| ALB087 | (AGC)7 | 192–202 | 14 | 0.00355 | 2.909 | 0.000 | 0.000 | 0.520 | 1.000 | 1.000 | 1.000 |
| ALB139 | (AGA)7 | 308–310 | 14 | 0.00495 | 1.999 | 0.000 | 0.000 | 0.459 | 1.000 | 1.000 | 1.000 |
| ALB165 | (ATA)12 | 173–201 | 18 | 0.00369 | 4.745 | 0.000 | 0.099 | 0.776 | 0.873 | 1.000a | 1.000 |
| ALB181 | (AC)9 | 234–238 | 14 | 0.00437 | 1.983 | 0.000 | 0.000 | 0.337 | 1.000 | 1.000 | 1.000 |
| ALB311 | (GA)6 | 244–250 | 14 | 0.00152 | 2.649 | 0.000 | 0.000 | 0.357 | 1.000 | 1.000 | 1.000 |
| Overall | - | - | 15 | 0.00383 | 3.023 | 0.002 | 0.025 | 0.507 | 0.952 | 0.922 | 0.987 |
Na: total number of alleles; pnull: average frequency of null alleles across populations; AR: average allelic richness; Hs: expected within population Nei’s heterozygosity; Ho: observed within population Nei’s heterozygosity; HT: expected Nei’s heterozygosity within the total population; Gst: the Nei’s measure of genetic differentiation; FIS: inbreeding coefficient estimated in FSTAT (a indicates values deviating from HWE); FIS §: inbreeding coefficient estimated using the Bayesian procedure implemented in INEst)