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. 2017 Jun 15;18:30. doi: 10.1186/s12865-017-0214-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of priming, pentraxin concentration and pentraxin ligands on IL-10 production. PBMC were cultured in (a-c) M-CSF or d-f) GM-CSF for 6 days and then with increasing concentrations of (a, d) SAP, b, e) CRP, or c, f) PTX3, in the presence or absence of factor H (100 μg/ml), MBL (2 μg/ml), or C1q (30 μg/ml), for an additional two days. Supernatants were then collected from the cells and assessed by ELISA for IL-10. Insert shows IL-10 production by SAP alone. Values are mean ± SEM (n = 8 SAP for M-CSF; n = 9 CRP for M-CSF; n = 9 PTX3 for M-CSF, n = 5 SAP for GM-CSF; n = 9 CRP for GM-CSF; n = 5 PTX3 for GM-CSF per group). *p < 0.05 (1-way ANOVA with Dunn’s test)