Fig. 7.

Effect of pentraxin concentration and pentraxin ligands on monocyte differentiation. PBMC were cultured in for 6 days with increasing concentrations of (a, d, g) SAP, (b, e, h) CRP, or c, f, i) PTX3 in the presence or absence of factor H (100 μg/ml), MBL (2 μg/ml), or C1q (30 μg/ml). Cells were then air-dried, fixed, and stained by ICC with antibodies against (a-c) CD163, (d-f) CD169, and (g-i) CD206. Results show the percent positive macrophages expressed as the mean±SEM (n = 4 separate donors)