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. Author manuscript; available in PMC: 2017 Jun 16.
Published in final edited form as: Am J Transplant. 2010 Sep 14;10(12):2586–2595. doi: 10.1111/j.1600-6143.2010.03257.x

Figure 4. Apoptosis and CD4+ T-cell infiltrate are reduced in hCD39 kidneys after IRI.

Figure 4

(A) Sections of renal tissue were analyzed by TUNEL-stain and the number of apoptotic nuclei per high power field (hpf) were counted. Results are presented as mean ± SEM. N.S (not significant). (B) Representative sections of TUNEL-stained renal cortex at 24 and 48 h. Magnification 400×. (C–F) Mice were subjected to 30 min of ischemia and 3 h of reperfusion. Leukocytes were extracted from the kidneys and analyzed by flow cytometry. (C) CD4+ T cells. (D) Neutrophils. (E) Macrophages. (F) MPO activity assay was performed following 3 h of reperfusion. Data are representative of two independent experiments; n = 4 for each group in each experiment.