Figure 6.

Autophagy induction observed in vivo. (a) Representative images of muscle tissue sections from GFP-LC3 mice treated with vehicle or peptide at 20 mg/kg for Tat-Beclin 1, 15 mg/kg for Tat-11mer and its analogues, and 15 mg/kg and 30 mg/kg for DD5-o. Tissue was analyzed 6 h after intraperitoneal injection of peptide. (b) GFP-LC3 puncta counted per 2500 μm² of muscle tissue. A minimum of ten fields was counted per tissue section. Bars represent mean ± SEM for four mice. * denotes P < 0.05 and ** denotes P < 0.01 by t test for indicated group vs DMSO control.