Fig. 1.

CBX3-UCOE-mediated transgene expression in hiPSC-derived macrophages. A Scheme of the lentiviral vector expressing GFP from the EFS (Lv.CBX3.EFS.GFP) (LTR: long terminal repeat). The lentiviral vector was further equipped with a CBX3-UCOE to prevent epigenetic transgene silencing such as methylation and to favor an open chromatin state. B Experimental scheme of lentiviral-mediated transduction of hiPSCs. Lentiviral particles (Lv.CBX3.EFS.GFP) were used to transduce hiPSC monolayer. After transduction, modified iPSCs were subjected to hematopoietic differentiation to study transgene stability. C Flow-cytometric analysis of GFP in transduced CD34iPSC11 over 28 days of culture. D GFP expression in transduced CD34iPSC16 and during hematopoietic differentiation in EBs and MCFCs (scale bars; column 1 and 3: 500 µm, column 2 and 4: 200 µm). E GFP expression in CD34iPSC16-derived macrophages in cell culture (left column) and unstained cytospin (right column) (scale bars: 100 µm). F Flow-cytometric analysis and G stained cytospins of terminally differentiated macrophages.