Fig. 2.

Stable transgene expression from the AAVS1 locus in hiPSC-derived macrophages. A Scheme of ZFN-mediated targeting of the fluorescence reporter genes GFP or RedStar_nuc into the AAVS1 safe harbor locus (HAL, homology arm left; HAR, homology arm right). B Schematic outline of nuclease-mediated genome editing of iPSCs. After nucleofection of iPSCs with suitable ZFNs and donor constructs, GFP- or RedStar_nuc-positive cells were sorted to obtain transgenic iPS single cell clones. C GFP expression during hematopoietic differentiation (scale bars; column 1 and 2: 200 µm, column 3 and 4: 500 µm) and E in iPSC-derived macrophages (scale bars: 100 µm). D RedStar_nuc expression during hematopoietic differentiation (scale bars; column 1 and 2: 200 µm, column 3 and 4: 500 µm) and F in iPSC-derived macrophages (scale bars; column 1: 100 µm and column 2: 50 µm). G Flow-cytometric analysis of terminally differentiated macrophages derived from iPSCs which either express GFP or H RedStar_nuc from the AAVS1 locus. I Cytospins of differentiated macrophages derived from iPSCs which either express GFP or J RedStar_nuc.