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GeNorm determination of optimal number of reference genes, indicated by a GeNorm V value. This algorithm analyzes the stability when using multiple reference genes, and uses a cut-off value of 0.15 (indicated by a red line). When comparing the stability of 6 reference genes over 5 (V5/6), the value is 0.15, which means that there is no additional value of the usage of 6 reference genes. The most optimal number of reference genes is therefore 5
