Fig. 5.

Runx1^hi population contains the main hematopoietic colony-forming activity. A After exclusion of CD140b+ OP9 cells, CD41^lowRunx1^neg or CD41^hiRunx1^hi were purified by flow cytometry. The percentages of CD41+ and CD45+ cells pre-sort are indicated. B 500 cells of each purified population were tested for the presence of hematopoietic colony forming cells in triplicate. Columns represent the total number colonies formed after 12 days per 500 cells seeded. The mean proportions of different colony types are depicted ± SD. M = CFU-monocyte/macrophage; G = CFU-granulocyte; CFU-E = erythroid colony forming units; GM = CFU-granulocyte-monocyte/macrophage; GEMM = CFU-granulocyte/erythroid/monocyte-macrophage/megakaryocyte.