TABLE 2.

tlyA mutations in spontaneous capreomycin-resistant M. tuberculosis mutants

Straina	Mutation site (base no.)	Nucleotide change	Amino acid change	MIC (μg/ml)b
				CAP	VIO
H37Rvc
C-201	758	ΔC	Frameshift	40	20
C-202	7	C→T	Arg 3 Stop	40	20
C-203	397	C insertion	Frameshift	40	20
C-206	555	T→G	Phe 185→Leu
C-210	353	T→C	Leu 118 Pro	40	40
C-211	64	C→T	Gln 22 Stop
C-212	52	C→T	Arg 18 Stop
C-213	383	T→A	Val 128 Glu
C-214	26	ΔC	Frameshift
C-215	200	C→A	Ala 67 Glu
C-216	None	N/A	N/A
CDC1551c
C-302	550	C→T	Gln 184 Stop
C-307	64	C→T	Gln 22 Stop
C-308	586	ΔG	Frameshift	40	20
C-309	550	C→A	Gln 184→Lys
C-310	653	ΔT	Frameshift
C-311	550	C→T	Gln 184 Stop
C-312	64	C→T	Gln 22 Stop	40	≤10
C-313	310	ΔG	Frameshift
C-314	205	A→G	Lys69→Glu
C-315	548	C→T	Pro183→Leu	40	20
C-316	400	ΔA	Frameshift
Beijing Fc
C-501	272	C→A	Ala91→Glu	40	≤10
C-502	712	G→A	Glu238→Lys
C-503	673-674	ΔGΔT	Frameshift
C-504	550	C→T	Gln 184 Stop	40	≤10
C-505	477	ΔG	Frameshift	40	≤10
C-507	550	C→T	Gln 184 Stop
C-509	550	C→T	Gln 184 Stop
C-510	None	N/A	N/A

^aMutants were selected on 7H10 containing 10μg of capreomycin/ml.

^bMICs were determined for a missense, nonsense, and frameshift mutant from each of the three different parent strains.

^cAll parent strains were susceptible to 10 μg of capreomycin/ml and 10 μg of viomycin/ml. CAP, capreomycin; VIO, viomycin; N/A, not applicable.