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. 2017 Jul;23(7):1060–1067. doi: 10.1261/rna.061226.117

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© 2017 Deryusheva and Gall; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Vertebrate guide RNAs for U2 snRNA pseudouridylation at position 43. (A) Predicted base-pairing with the U2 snRNA branch point recognition region. Arrows indicate nucleotides in U2 snRNA that differ between yeast and higher eukaryotes. (B,C) Testing guide RNA activities in a yeast cell system. (B) Modification of yeast U2 snRNA mediated by Drosophila scaRNA:ΨU2-35.45, mouse SCARNA8, Xenopus SNORA71, and pugU2-43 guide RNAs expressed in the pus7Δ, pus1Δ, and pus1Δsnr81Δ strains. (C) Modification of the vertebrate branch point recognition region (nucleotides 29–52) inserted into an artificial U87-derived RNA, mediated by mouse SCARNA8 and Drosophila scaRNA:ΨU2-35.45 guide RNAs expressed in the pus1Δ strain. Stars indicate the guide RNA-induced modifications.