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. 2017 Jul;23(7):1060–1067. doi: 10.1261/rna.061226.117

FIGURE 4.

FIGURE 4.

S. pombe guide RNA for U2 snRNA pseudouridylation at positions 43 and 44. (A) Scheme of the endogenous gene and expression constructs for S. pombe pugU2-43/44 RNA. (B) Predicted structural alterations and base-pairing with U2 snRNA within the 3′-terminal pseudouridylation pocket of sppugU2-43/44 RNA. Star indicates uridine in the upper stem that was deleted in the mutant variant sppugU2-43/44ΔUmut. (C) Northern blot analysis of endogenous expression of pugU2-43/44 RNA in S. pombe strains and exogenous expression from plasmids (two constructs shown in A) in S. cerevisiae. All samples were run on the same gel and probed simultaneously. The first three lanes are shown with a longer exposure than the remaining four lanes. (D) Probing the modification guide RNA activities of sppugU2-43/44 in the BY4741 wild-type and the pus1Δ mutant strains of S. cerevisiae. Stars indicate modifications that are induced by sppugU2-43/44 RNA expression.