FIGURE 3.
C-terminal dsRBD of Dicer-2 is important for high-fidelity 21-nt siRNA production in vivo. Sequencing results of small RNAs prepared from female fly heads are shown. The reads were normalized by the sequencing depth. (A) Normalized number of reads of 19- to 23-nt wIR-derived siRNAs. (B) Abundance and 5′ position of wIR-derived siRNAs. Antisense siRNAs are shown in red, sense in blue. Graphs on the right have different y-axis scales. (C) Length distribution of wIR-derived siRNAs. Data for the control, dicer-2null, wild-type rescue, phosphate-binding pocket mutant are from Kandasamy and Fukunaga (2016).