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Both N-terminal helicase domain and C-terminal dsRBD of Dicer-2 are important for siRNA loading to Argonaute2 in ovary lysate in vitro. (A) Representative gel image of in vitro siRNA loading assay using ovary lysate. (B) Quantification of the signals in the gels normalized to the background signals in the ovary lysate of dicer-2null without any rescue gene (negative control). Data are mean ± SD for four independent trials. (*) P-value <0.05.
