Figure 1. Cell line based model of melanoma progression and epigenome profiling.

(A) Brief description of the primary melanocyte based model system that consists of two replicates of paired isogenic non (or weakly)-tumorigenic (NTM_H, NTM_P) and tumorigenic (TM_H and TM_P) cells. Kaplan-Meier curve showing tumor formation efficiency of NTM_H, NTM_P, TM_H and TM_P cells. NTM_H and NTM_P cells display long latency whereas TM_H and TM_P cells show shorter latency for tumor formation. Mantle-Cox p = .0007 for NTM_H vs TM_H and p = 0.0016 for NTM_P vs TM_P (B) Proliferation curve showing differences in cell confluence (Y-axis) in NTM_H vs TM_H and NTM_P vs TM_P as a function of time (X-axis). (C) Normalized signal of all profiled chromatin marks, IgG control and RNA-Seq in an example region (chr10:43,572,517-44,100,000) for NTM_H (blue) and TM_H (red) cells. Chromatin state tracks and gene annotations are also shown. (D) Log₂ ratio between NTM_H and TM_H cells for the average signal strength of each chromatin mark in a window of 2kb around annotated transcription start sites from RefSeq (Green) and on DNaseI hypersensitive sites from ‘Melano’ (Purple) cell lines from ENCODE. See also Figure S1 and Table S1.