Figure 6. CBP loss in NTM_H cells promotes tumorigenesis and mimics acetylation loss seen in TM_H cells.

(A) Bar graph showing relative levels of 32 histone acetyltransferases and deacetylases between NTM_H/TM_H and NTM_P/TM_P cells. The Y–axis shows Log₂ Fold Change values. The dotted line shows the cutoff of 2-fold change. (B-C) Graph showing relative levels of CBP histone acetyltransferase in (E) NTM_H, TM_H, NTM_P and TM_P cells and (F) NTM_H cells harboring either control or CBP shRNAs. (D-G) Boxplots showing average normalized intensity for ChIP-string probes for (D, F) H2BK5Ac and (E, G) H4K5Ac in NTM_H, TM_H, NTM_H cells harboring CBP shRNAs or NRAS^G12D expressing transformed melanocytes (M-NRAS). The plot is limited to those probes that were originally enriched in (D-E) NTM_H cells or in (F-G) in TM_H cells by ChIP-Seq experiments and validated by ChIP-String in Figure S2A-F. Asterisk (*) represents p<0.05 and double asterisk (**) represents p<0.001 (Wilcoxon Rank test) when comparisons are made to NTM_H. (H) Kaplan-Meier curve showing tumor formation efficiency of NTM_H cells harboring control or CBP shRNAs (shCBP-1 and shCBP-2).