Figure 7. Acetylation status on deacetylated promoters in T_H correlates with response to HDAC inhibitors. (A-B).

Boxplots showing average normalized intensity for (A) H2BK5Ac or (B) H4K5Ac on ChIP-string probes (that were enriched in NTM_H cells by ChIP-Seq experiment) across NTM_H and TM_H cells that were either untreated or treated with vorinostat (200nM) or entinostat (300nM) for 72hrs. Asterisk (*) represents p<0.05 and double asterisk (**) represents p<0.001 (Wilcoxon Rank test) when comparisons are made to TM_H. (C-D) Growth curves for NTM_H and TM_H cells grown under various concentrations of (C) vorinostat or (D) entinostat. (E) Aggregate plot showing H3K27Ac levels around +/-2Kb of deacetylated gene promoters (in T_H cells) in various melanoma cell lines. (F) Growth curves for melanoma cell lines grown under various concentrations of vorinostat. (G) Table showing IC₅₀ values (the concentration at which 50% response is achieved) and area under the curve (AUC) for two HDAC inhibitors, vorinostat and entinostat, in melanoma cells lines. Immmeasurable IC₅₀ values are shown as NaN which refers to ‘not a number’. (H) Correlation plot between AUC and average H3K27Ac levels at TSS of gene promoters that showed loss of histone acetylation in TM_H cells compared to NTM_H cells. See also Figure S7.