Figure 1.
Phrenic motor neuron survival at end stage in SOD1G93A rats. A, B, Photomicrographs of NeuN staining (green) in putative phrenic motor neurons from an end-stage SOD1G93A rat (B) and wild-type littermate (A). The phrenic motor nucleus is circumscribed in each panel, and is shown at higher magnification in the inset in each panel. C, D, Number of surviving C4 NeuN+ putative phrenic motor neurons were counted and compared between siRNA-treated (C) and UO126-treated and PI828-treated (D) SOD1G93A rats (dark gray bars) compared with wild-type (WT) littermates (light gray bars). Phrenic motor neuron counts were lower in SOD1G93A rates compared with age-matched WT rats (*p < 0.001). Scale bars: (in B) A, B, 200 μm; higher-magnification insets (in B) A, B, 50 μm. Values are means ± 1 SEM.