Figure 6.
Modulation of protein dynamics imparted by covalent modifications of ArCP. A–C, 3D representation of significant variations resulting from phosphopantetheinylation (A), salicylate (Sal) attachment (B), and combined effect of S-salicylyl-phosphopantetheine (C). The thickness of the sausage reflects the magnitude of differences in order parameters (H–J, corresponding to A–C, respectively), with a thicker line emphasizing residues varying by more than one S.D. value from the mean, accounting for errors (shown with black dots in H–J). The color, from pale to dark, reports on changes in Rex. D–F, 3D representations of the dynamics of apo-ArCP (D), holo-ArCP (E), and loaded ArCP (F). G, apparent allosteric effect resulting from the modulation of dynamics shown in A–C. H–J, differences in order parameters, S2, used to generate the 3D representations in A–C. Dashed horizontal lines, limits for one S.D., used to emphasize residues with significant changes.