Table 1.
ITC-derived thermodynamic parameters of YbtE binding to all forms of ArCP
Binding constants (KD), enthalpy (ΔH), entropy (ΔS), and stoichiometry (n) were determined by fitting experimental data to a one-site binding model.
KD | ΔH | ΔS | n | |
---|---|---|---|---|
μm | kcal/mol | cal/mol/K | ||
Apo-ArCP | 11.6 ± 1.9 | 0.62 ± 0.04 | 24.6 ± 3.7 | 1.32 ± 0.05 |
Holo-ArCP | 2.7 ± 0.4 | −3.50 ± 0.08 | 13.8 ± 3.5 | 1.09 ± 0.02 |
SalNH-ArCP | 1.9 ± 0.2 | −1.42 ± 0.02 | 21.5 ± 2.5 | 0.96 ± 0.01 |